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Estuarine ecological risk based on hepatic histopathological indices from laboratory and in situ tested fish
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Orientador(es)
Resumo(s)
Juvenile Senegalese soles were exposed through 28-day laboratory and field (in situ) bioassays to sediments
from three sites of the Sado estuary (W Portugal): a reference and two contaminated by metallic
and organic contaminants. Fish were surveyed for ten hepatic histopathological alterations divided by
four distinct reaction patterns and integrated through the estimation of individual histopathological condition
indices. Fish exposed to contaminated sediments sustained more damage, with especial respect to
regressive changes like necrosis. However, differences were observed between laboratory- and fieldexposed
animals, with the latest, for instance, exhibiting more pronounced fatty degeneration and hepatocellular
eosinophilic alteration. Also, some lesions in fish exposed to the reference sediment indicate
that in both assays unaccounted variables produced experimental background noise, such as hyaline
degeneration in laboratory-exposed fish. Still, the field assays yielded results that were found to better
reflect the overall levels of contaminants and physico-chemical characteristics of the tested sediments.
Descrição
Palavras-chave
Solea senegalensis Histopathology Weighted indices Contaminated sediments Estuary Bioassays