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- Bioinformatic tools in the study of ancient dogs: preliminary results of an Iberian case studyPublication . Blaschikoff, Ludmilla; Serra, Octávio; Guimarães, Silvia; Cardoso, João Luís; Simões, Fernanda; Detry, Cleia; Ginja, Catarina; Fernández-Rodríguez, Carlos; Ferreira, Eduardo; Pires, Ana ElisabeteIn this study, we attempted to carry out a genomic amplification of ancient DNA from four pre-historic Iberian dogs using the Illumina sequencing method; to assign mtDNA sequences to the major dog haplogroups and to characterize some phenotypic traits of these dogs.
- Unraveling the genomes of ancient Iberian CanisPublication . Blaschikoff, Ludmilla; Serra, Octávio; Cardoso, João Luís; Fernández-Rodríguez, Carlos; Sousa, Ana Catarina; Moreno-García, Marta; Guimarães, Sílvia; Simões, Fernanda; Detry, Cleia; Gotherstrom, Anders; Ginja, Catarina; Pires, Anabela Elisabete
- The curious case of the Mesolithic Iberian dogs: an archaeogenetic studyPublication . Pires, Ana Elisabete; Detry, Cleia; Chikhi, Lounes; Rasteiro, Rita; Amorim, Isabel R.; Simões, Fernanda; Matos, José; Petrucci-Fonseca, Francisco; Ollivier, Morgane; Hänni, Catherine; Cardoso, João Luís; Arias, Pablo; Diniz, Mariana; Araújo, Ana Cristina; Bicho, Nuno; Sousa, Ana Catarina; Moreno-García, Marta; Arruda, Ana Margarida; Fernández-Rodríguez, Carlos; Porfírio, Eduardo; Arnaud, José Morais; Valente, Alexandra; Gonçalves, David; Alves, Lara; Götherström, Anders; Davis, Simon J. M.; Ginja, CatarinaWe investigated the genetic, composition of six Canis remains from western Iberia, directly radiocarbon dated to 7,903-7,570 years (cal BP). They were identified as dogs via their qrchaeological and depositional context, osteometry, and a high percentage of aquatic diet shared with humans. For comparison, genetic data were obtained from an additional 37 Iberian dog remains from the Neolithic to Late Antiquity, as well as two Palaeolithic anda a Chalcolithic Canis identified as wolves. Previous data indicated that dog mtDNA haplogroup A (HgA) is prevalent in extant European dogs (>50%), in the Near East and Asia, but rare or absent (<10%) in European Canis older than 3,000 years (cal BP). We found a high frequency of dog HgA in pre-Neolithic Europe. We show that, contrary to the current view, Canis with HgA did not necessarily arrive in Europe from East-Asia. This phylogeographical difference in HgA frequency demonstrates that genetic differentiation was prior to, or as a consequence of, domestication which may be linked with pre-Neolithic local processes for Iberian wolf domestication. Our results emphasize that knowledge of both ancient wolves and early dogs genetic profiles from the European periphery should improve our understanding of the evolution of the European dog.
- Iberian Chalcolithic Canis: a genomic approach to know them betterPublication . Blaschikoff, Ludmilla; Serra, Octávio; Guimarães, Sílvia; Cardoso, João Luís; Simões, Fernanda; Detry, Cleia; Ginja, Catarina; Fernández-Rodríguez, Carlos; Ferreira, Eduardo; Pires, Anabela ElisabeteTo our knowledge, for Iberian ancient dogs and wolves, this is the first attempt to successfully apply NGS methods.
- A multidisciplinary study of Iberian Chalcolithic dogsPublication . Blaschikoff, Ludmilla; Daza-Perea, Arantxa; Requicha, J. F.; Detry, Cleia; Rasteiro, Rita; Guimarães, Sílvia; Ureña, Irene; Serra, Octávio; Schmidt, Ryan; Valera, António Carlos; Almeida, Nelson J.; Porfírio, Eduardo; Santos, Ana Beatriz; Delicado, Cátia; Simões, Fernanda; Matos, José; Rosário, Isabel Amorim do; Petrucci-Fonseca, Francisco; Davis, Simon J. M.; Muñoz Merida, Antonio; Gotherstrom, Anders; Fernández-Rodríguez, Carlos; Cardoso, João Luís; Ginja, Catarina; Pires, Ana ElisabeteDomesticated dogs have been present in the Iberian Peninsula long before other domesticated species, back to the late Palaeolithic period. Their origin is still uncertain, but dogs were already well established during the Chalcolithic period (ca. 5000–4000 BP). This study employed a multidisciplinary approach comprising osteometric, radiographic and palaeogenomic analyses to characterize Chalcolithic Iberian Canis remains. Two Chalcolithic archaeological sites – Leceia, Oeiras, in Portugal, and El Caset´on de la Era, Villalba de los Alcores, Valladolid, in Spain – were the main focus of this study. Osteometric and odontometric data from eleven other sites in Iberia were also included. Osteometric results show signs of phenotypic variability, likely the result of human-driven selective pressure. Dental radiographic and dental wear analyses allowed age at death estimation for four individuals (two juvenile and two adults). Three Chalcolithic Iberian dogs had their mitogenomes resequenced and the mitochondrial DNA analysis allowed to assign each individual to two of the major known haplogroups – A and C. Molecular sex infered by the chromosomeX/chromosome1 coverage ratio allowed to identify one female and two males. This study unveils some aspects of the Iberian Chalcolithic dogs: these dogs already exhibited various morphotypes whose profiles might be associated to the performance of certain tasks, as well as mitogenomes of two distinct lineages that help tracking the evolutionary paths of Iberian dogs.