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Assessment of the genotoxic hazard of estuarine sediments using an integrative approach with LacZ Plasmid-Based transgenic mice

2025-06-16Artigo científico Acesso restrito
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dc.contributor.authorPinto, Miguel
dc.contributor.authorSacadura, Joana
dc.contributor.authorCosta, Pedro M.
dc.contributor.authorCaeiro, Sandra
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria J.
dc.date.accessioned2026-01-16T11:33:43Z
dc.date.available2026-01-16T11:33:43Z
dc.date.issued2025-06-16en_US
dc.date.updated2025-10-24T08:31:23Z
dc.description.abstractUnder the influence of multiple anthropogenic pressures, from industrial to agricultural activities, estuaries have long been regarded as particularly sensitive ecosystems to contamination. The present study aimed at investigating the genotoxic potential of a contaminated sediment sample from an urban and industrial area of the Sado Estuary, by combining the analysis of multiple endpoints in the LacZ plasmid-based transgenic mouse model exposed for 28 days to contaminated estuarine sediment extracts through drinking water. The DNA and chromosome damaging effects were monitored in peripheral blood at 7-day intervals using the standard and enzyme-modified Comet assay, as well as the micronucleus assays in peripheral blood cells. After euthanasia, DNA damage was analyzed in several mouse tissues, and LacZ mutant frequencies were determined in the liver. Livers were also surveyed for histopathological analysis. A time-dependent increase in micronuclei frequency was seen at all tested doses, in spite of no induction of DNA damage in any organ or mutation induction in the liver of exposed mice. The liver from mice exposed to sediment extracts did not reveal major alterations besides evidence of inflammation. Overall, the integration of the endpoints analyzed in the mice is suggestive of potential chronic, rather than acute, adverse effects in vivo, and points to the need for further research in the resident human population in the area. This experimental design can be used to assess the genotoxicity of complex environmental mixtures, understand how they work, and reduce costs and resources while speeding up data collection and interpretation.eng
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e a Tecnologia, LA/P/0140/2020, PTDC/SAU-ESA/100107/2008, SFRH/BPD/72564/2010, UIDP/04378/2020, UIDB/04378/2020.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1002/em.70019en_US
dc.identifier.issn0893-6692en_US
dc.identifier.issn1098-2280en_US
dc.identifier.slugcv-prod-4586317
dc.identifier.urihttp://hdl.handle.net/10400.2/20901
dc.language.isoeng
dc.peerreviewedyes
dc.relationInstitute for Health and Bioeconomy
dc.relationHERA - Environmental Risk Assessment of a contaminated estuarine environment: A case study
dc.relationANALYSIS OF THE GENOTOXIC POTENTIAL OF ESTUARINE SEDIMENTS AND THE DNA REPAIR AND APOPTOSIS-RELATED RESPONSES IN HUMAN HEPG2 CELLS
dc.relationApplied Molecular Biosciences Unit
dc.rights.uriN/A
dc.subjectEstuary sediments
dc.subjectGenotoxicity
dc.subjectHistopathology
dc.subjectLacZ mice
dc.subjectMutant frequency
dc.titleAssessment of the genotoxic hazard of estuarine sediments using an integrative approach with LacZ Plasmid-Based transgenic miceeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleHERA - Environmental Risk Assessment of a contaminated estuarine environment: A case study
oaire.awardTitleANALYSIS OF THE GENOTOXIC POTENTIAL OF ESTUARINE SEDIMENTS AND THE DNA REPAIR AND APOPTOSIS-RELATED RESPONSES IN HUMAN HEPG2 CELLS
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIhttp://hdl.handle.net/10400.2/20898
oaire.awardURIhttp://hdl.handle.net/10400.2/20899
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F72564%2F2010/PT
oaire.awardURIhttp://hdl.handle.net/10400.2/20900
oaire.citation.endPage310
oaire.citation.issue6-7en_US
oaire.citation.startPage300
oaire.citation.titleEnvironmental and Molecular Mutagenesisen_US
oaire.citation.volume66en_US
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
oaire.fundingStreamConcurso para Projectos de I&D em todos os Domínios Científicos - 2008
oaire.fundingStreamOE
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCaeiro
person.givenNameSandra
person.identifier587808
person.identifier.ciencia-id8515-398A-D241
person.identifier.orcid 0000-0002-6079-3554
person.identifier.ridK-3886-2014
person.identifier.scopus-author-id6603297853
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid8515-398A-D241 | SANDRA SOFIA FERREIRA DA SILVA CAEIRO
rcaap.rightsclosedAccessen_US
relation.isAuthorOfPublicatione2a8250a-8f09-4f11-a04e-1a3056644ff3
relation.isAuthorOfPublication.latestForDiscoverye2a8250a-8f09-4f11-a04e-1a3056644ff3
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