Publication
Leaching of pyrite by acidophilic, heterotrophic, iron-oxidizing bacteria in pure and mixed culture
| datacite.subject.sdg | 12:Produção e Consumo Sustentáveis | pt_PT |
| datacite.subject.sdg | 15:Proteger a Vida Terrestre | pt_PT |
| dc.contributor.author | Nicolau, Paula Bacelar | |
| dc.contributor.author | Johnson, D. Barrie | |
| dc.date.accessioned | 2023-06-15T12:59:52Z | |
| dc.date.available | 2023-06-15T12:59:52Z | |
| dc.date.issued | 1999 | |
| dc.description.abstract | Seven strains of heterotrophic iron-oxidizing acidophilic bacteria were examined to determine their abilities to promote oxidative dissolution of pyrite (FeS2) when they were grown in pure cultures and in mixed cultures with sulfur-oxidizing Thiobacillus spp. Only one of the isolates (strain T-24) oxidized pyrite when it was grown in pyrite-basal salts medium. However, when pyrite-containing cultures were supplemented with 0.02% (wt/vol) yeast extract, most of the isolates oxidized pyrite, and one (strain T-24) promoted rates of mineral dissolution similar to the rates observed with the iron-oxidizing autotroph Thiobacillus ferrooxidans. Pyrite oxidation by another isolate (strain T-21) occurred in cultures containing between 0.005 and 0.05% (wt/vol) yeast extract but was completely inhibited in cultures containing 0.5% yeast extract. Ferrous iron was also needed for mineral dissolution by the iron-oxidizing heterotrophs, indicating that these organisms oxidize pyrite via the “indirect” mechanism. Mixed cultures of three isolates (strains T-21, T-23, and T-24) and the sulfur-oxidizing autotroph Thiobacillus thiooxidans promoted pyrite dissolution; since neither strains T-21 and T-23 nor T. thiooxidans could oxidize this mineral in yeast extract-free media, this was a novel example of bacterial synergism. Mixed cultures of strains T-21 and T-23 and the sulfur-oxidizing mixotroph Thiobacillus acidophilus also oxidized pyrite but to a lesser extent than did mixed cultures containing T. thiooxidans. Pyrite leaching by strain T-23 grown in an organic compound-rich medium and incubated either shaken or unshaken was also assessed. The potential environmental significance of iron-oxidizing heterotrophs in accelerating pyrite oxidation is discussed. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.issn | 0099-2240 | |
| dc.identifier.uri | http://hdl.handle.net/10400.2/14053 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.subject | Environmental microbiology | pt_PT |
| dc.subject | Pyrite leaching | pt_PT |
| dc.subject | Bacterial consortia | pt_PT |
| dc.title | Leaching of pyrite by acidophilic, heterotrophic, iron-oxidizing bacteria in pure and mixed culture | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 590 | pt_PT |
| oaire.citation.issue | 65 | pt_PT |
| oaire.citation.startPage | 585 | pt_PT |
| oaire.citation.title | Applied and Environmental Microbiology | pt_PT |
| person.familyName | Nicolau | |
| person.givenName | Paula Bacelar | |
| person.identifier | https://scholar.google.com/citations?hl=pt-PT&user=WWFc0MkAAAAJ | |
| person.identifier.ciencia-id | 7D11-BE78-21FD | |
| person.identifier.orcid | 0000-0001-5940-5131 | |
| person.identifier.scopus-author-id | 6507462575 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | c65a3227-1c5b-4bd3-b49c-95c19d53fdd6 | |
| relation.isAuthorOfPublication.latestForDiscovery | c65a3227-1c5b-4bd3-b49c-95c19d53fdd6 |