Browsing by Author "Bustamante, Paco"
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- Accumulation of mercury in the tissues of the common octopus Octopus vulgaris (L.) in two localities on the Portuguese coastPublication . Seixas, Sónia; Bustamante, Paco; Pierce, GrahamMercury concentrations were measured in tissues of 12 individuals of Octopus vulgaris, captured by the commercial fishery at two points along the Portuguese coast, Viana do Castelo and Cascais, in spring 2002. Concentrations were determined in six tissues (digestive gland, branchial hearts, gills, mantle, arms, and gonads). Correlations between mercury concentrations in different tissues were examined as were correlations between mercury levels and total length, mantle length, weight, gonadosomatic index, digestive gland index, and state of maturation. Differences between sexes and localities were analysed. The concentration of mercury in the digestive gland (Viana, 0.58F0.08, and Cascais, 3.43F2.57 mg/kg dry weight) was higher than in the other tissues, and values were generally similar to those recorded in previous studies on octopods. Arm muscle contained most of the mercury with 56% of the total body burden followed by the digestive gland with 31%, mantle with 11%, gills with 0.8%, gonad with 0.5%, and branchial heart with 0.2%. In all tissues, mercury concentrations were slightly higher in samples from Cascais than in Viana do Castelo, which is consistent with higher concentrations recorded in seawater at Cascais. Levels of mercury determined in octopus were within the range of values legally defined as safe for human consumption.
- Diseases, parasites, and contaminantsPublication . Malham, Shelagh; Bustamante, Paco; Pascual, Santiago; Seixas, Sónia
- Interannual patterns of variation in concentrations of trace elements in arms of Octopus vulgarisPublication . Seixas, Sónia; Bustamante, Paco; Pierce, Graham
- Interannual patterns of variation in concentrations of trace elements in arms of Octopus vulgarisPublication . Seixas, Sónia; Bustamante, Paco; Pierce, GrahamConcentrations of essential (copper, iron, manganese, selenium, andzinc) andnon-essential (arsenic, cadmium, mercury, andlead ) trace elements were measuredin arms of Octopus vulgaris. The cephalopods were sampled from commercial fishery landings at two sites on the Portuguese coast in spring 2002 and 2003. Mercury was determined using an Advanced Mercury Analyser Spectrophotometer (AMAS) and other trace elements were measured using inductively coupled plasma-atomic emission spectrometry (ICP-AES). Since high levels of arsenic were detected, identification of the forms present was carriedout using high performance liquid chromatography (HPLC) followedby ICP-MS. Mean concentrations of trace elements analysedw ere in the following order: As > Zn > Fe > Cu Cd> Pb > Se >Mn Hg. The results of speciation of arsenic demonstrate that virtually all arsenic was in the arsenobetaine form, which is the less toxic form. The concentrations of several trace elements were generally high in samples from Viana in 2002. Cadmium concentrations were above the legal limit for human consumption in samples from Viana in 2002 and two of these animals alsohadleadconcentrations that exceededlegal limits.Mercury appearedin all samples but levelswerewithin legally defined safe limits.No relationshipwas detected between trace element concentrations and size ormaturity of octopus.
- The future of cephalopod populations, fisheries, culture, and research in EuropePublication . Pierce, Graham; Belcari, Paola; Bustamante, Paco; Challier, Laurence; Cherel, Yves; González, Ángel; Guerra, Ángel; Jereb, Patrizia; Koueta, Noussithé; Lefkaditou, Eugenia; Moreno, Ana; Pereira, João; Piatkowski, Uwe; Pita, Cristina; Robin, Jean‐Paul; Roel, Beatriz; Santos, M. Begoña; Santurtun, Marina; Seixas, Sónia; Shaw, Paul; Smith, Jennifer; Stowasser, Gabrielle; Valavanis, Vasilis; Villanueva, Roger; Wang, Jianjun; Wangvoralak, Sansanee; Weis, Manuela; Zumholz, Karsten
- The significance of cephalopod beaks as a research tool: an updatePublication . Xavier, José C.; Golikov, Alexey V.; Queirós, José P.; Perales-Raya, Catalina; Rosas-Luis, Rigoberto; Abreu, José; Bello, Giambattista; Bustamante, Paco; Capaz, Juan C.; Dimkovikj, Valerie H.; González, Angel F.; Guímaro, Hugo; Guerra-Marrero, Airam; Gomes-Pereira, José N.; Kubodera, Tsunemi; Laptikhovsky, Vladimir; Lefkaditou, Evgenia; Lishchenko, Fedor; Luna, Amanda; Liu, Bilin; Pierce, Graham; Pissarra, Vasco; Reveillac, Elodie; Romanov, Evgeny V.; Rosa, Rui; Roscian, Marjorie; Rose-Mann, Lisa; Rouget, Isabelle; Sánchez, Pilar; Sánchez-Márquez, Antoni; Seixas, Sónia; Souquet, Louise; Varela, Jaquelino; Vidal, Erica A. G.; Cherel, YvesThe use of cephalopod beaks in ecological and population dynamics studies has allowed major advances of our knowledge on the role of cephalopods in marine ecosystems in the last 60 years. Since the 1960’s, with the pioneering research by Malcolm Clarke and colleagues, cephalopod beaks (also named jaws or mandibles) have been described to species level and their measurements have been shown to be related to cephalopod body size and mass, which permitted important information to be obtained on numerous biological and ecological aspects of cephalopods in marine ecosystems. In the last decade, a range of new techniques has been applied to cephalopod beaks, permitting new kinds of insight into cephalopod biology and ecology. The workshop on cephalopod beaks of the Cephalopod International Advisory Council Conference (Sesimbra, Portugal) in 2022 aimed to review the most recent scientific developments in this field and to identify future challenges, particularly in relation to taxonomy, age, growth, chemical composition (i.e., DNA, proteomics, stable isotopes, trace elements) and physical (i.e., structural) analyses. In terms of taxonomy, new techniques (e.g., 3D geometric morphometrics) for identifying cephalopods from their beaks are being developed with promising results, although the need for experts and reference collections of cephalopod beaks will continue. The use of beak microstructure for age and growth studies has been validated. Stable isotope analyses on beaks have proven to be an excellent technique to get valuable information on the ecology of cephalopods (namely habitat and trophic position). Trace element analyses is also possible using beaks, where concentrations are significantly lower than in other tissues (e.g., muscle, digestive gland, gills). Extracting DNA from beaks was only possible in one study so far. Protein analyses can also be made using cephalopod beaks. Future challenges in research using cephalopod beaks are also discussed.