Fontoura, Bruno H.Dallacorte, Lucas V.Simon, Anna P.Marchese, Amanda F.Rodrigues, Michelle F. F.Lima, Vanderlei A. deFernandes, TiagoCarpes, Solange T.Marchese, José A.2026-06-012026-06-012026-03-02Bruno H. Fontoura, Lucas V. Dallacorte, Anna P. Simon, Amanda F. Marchese, Michelle F.F. Rodrigues, Vanderlei A. de Lima, Tiago A. Fernandes, Solange T. Carpes, José A. Marchese, Chemometric Optimization of Functional Compound Extraction from Artemisia annua L. and Assessment of Its Antioxidant and Antimicrobial Properties, ACS Agric. Sci. Technol., 2026, 6, 716-731. https://doi.org/10.1021/acsagscitech.5c006292692-1952http://hdl.handle.net/10400.2/22094Artemisia annua L. is a medicinal plant valued for its bioactive compounds, especially artemisinin. This study focused on improving the extraction of these compounds using a CCRD design, combining ethanol/water (0−100%) and extraction times (60−300 s), with a mechanical homogenizer (ultradisperser). The obtained extracts were analyzed by using spectrophotometric methods (UV−vis and HPLC-DAD) to determine the presence of phenolic compounds, flavonoids, and sesquiterpenes. Antioxidant and antimicrobial capacities were evaluated. Ethanol concentrations between 50 and 60% increased the extraction of phenolics with antioxidant potential, and concentrations greater than 70% were more effective for sesquiterpenes. Extracts with a higher artemisinin content showed strong antimicrobial activity, especially against Gram-positive bacteria. Multivariate analyses validated the relationships between the extracted compounds and their biological effects. The overall extraction condition was 50% (ethanol/ water, v/v) for 180 s, highlighting the biological potential of A. annua L. and the importance of optimizing extraction processes.engMedicinal plantsArtemisininResponse surface methodologyCentral Composite Design (CCD)Statistical modelingjournal article10.1021/acsagscitech.5c00629